PRECLINICAL RESEARCH

Radiant Research with its strong background of Preclinical studies offers both in vitro (cell/microbe based) and In vivo (animal based) customised research services to evaluate the efficacy and safety of lead drugs/formulations before proceeding for clinical trials.
At Radiant Research, we offer customized preclinical efficacy and toxicology services to Pharma, Herbal, Ayurvedic, nutraceutical and cosmetic industry and have become favorite one stop destination for all drug discovery requirements.

Cancer

In vitro

  • Cytotoxicity assay by MTT/SRB/Trypan blue
  • Clonogenic assay
  • Apoptosis – Nuclear staining with DAPI
  • Alkylating activity assay
  • TUNEL assay
  • Endonuclease assay
  • DNA fragmentation analysis / DNA ladder assay
  • Cancer specific gene expression analysis
  • (P53, BCl2, Capsase 3, VEGF, cyclin D1, cyclin B1, STAT3 etc.)

In vivo

  • DLA/EAC induced ascetic tumor model in mice
  • DLA induced solid tumor model in mice

Diabetes

In vitro (cell based)

  • Glucose uptake studies in skeletal muscle cell lines (Non-radio labeled assay).
  • Glucose uptake in isolated rat hemi diaphragm
  • Gene expression studies (PPARγ, GLUT-4 etc)
  • Alpha-Amylase inhibitory activity
  • Alpha-galactosidase inhibitory activity
  • Alpha-glucosidase activity

In vivo (Animal based)

  • Alloxan induced diabetic rat model
  • Streptozotocin induced diabetic rat model

Hepatoprotective

In vitro (Cell based)

  • Hepatoprotective study against selected toxicant.
  • Hepatoprotective study in isolated primary rat hepatocytes.
  • Gene expression studies

In vivo (Animal based)

  • Hepatoprotective study against selected toxicant in rats/mice

Anti-infectives

In vitro 

  • Anti microbial activity by Zone of inhibition.
  • Anti microbial activity by MIC determination
  • Antibiotic sensitivity testing
  • TLC bioautography
  • In vitro anti viral studies

In vivo

  • Different wound healing models in mice/rats

Cosmetic research

EFFICACY

  • Skin Lightening
  • Sun Protection Factor (SPF)
  • Anti-aging
  • Skin tightening
  • Psoriasis
  • Scar/wound healing
  • Anti-acne
  • Anti-Dandruff
  • Deodorant
  • Skin Moisturizing
  • Skin pigmentation
  • Hair growth/anti-hair fall
  • Dermal permeation/absorption using EpiskinTM / EpidermTM/ Porcine skin/Start-M

SAFETY/TOXCITY

  • 3T3 NRU phototoxicity (Skin irritation)
  • Acute Dermal toxicity
  • Acute ocular toxicity
  • Acute inhalation toxicity
  • Skin sensitization
  • Genotoxicity
  • AMES test (BRMT)
  • Chromosomal aberration in CHO-K1 cells

Antioxidant

In vitro

  • DPPH scavenging assay
  • Scavenging of Nitric Oxide radical
  • Lipid peroxidation inhibitory activity
  • Evaluation of Total Antioxidant capacity
  • Ferric ion reducing power assay
  • Scavenging of Hydroxyl Radical by p-NDA Method
  • Scavenging of Hydrogen Peroxide
  • Scavenging of ABTS radical cation Assay
  • Total Phenolic and Flavonoid content estimation

In vivo

  • Antioxidant status in rats by hepatic injury model
  • Antioxidant status in tumor bearing mice model

Immunomodulatory & Inflammation

In vitro (cell based)

  • Phagocytic assay on cellular lysosomal enzyme activity
  • Proliferation assay
  • NO and TNF inhibitory studies
  • HRBC membrane stabilization
  • Inhibition of protein denaturation
  • Proteinase inhibitory action
  • Analysis of markers like COX-1, COX-2, PGE-2, iNOS, IFN-alpha, IFN-gamma, TNF-alpha, Interleukin (IL)-1, IL-10, IL12, IL-18 etc.

In vivo (Animal based)

  • Carbon clearance assay model
  • Cellular Immune Response Model
  • Humoral Immune Response Model
  • Neutrophil Adhesion Test Model
  • Radiant heat method
  • Tail immersion test
  • Writhing test
  • Paw edema model
  • Oxazolone-induced ear edema in mice

Obesity

In vitro 

  • Anti adepogensis studies in 3T3L-1 pre adepocytes by Oil Red-O staining
    Cell triglyceride assay
  • Gene expression studies (PPAR-gamma, SREBF etc)

In vivo

  • Triton induced hyperlipidemia model in mice/rats
  • Cocktail induced hypercholesterolemia model

Toxicology

  • Acute oral Toxicity
  • Acute Dermal toxicity
  • Acute ocular toxicity
  • Acute inhalation toxicity
  • Repeat-Dose Toxicity Studies (Sub-acute/Subchronic/Chronic)
  • Skin sensitization
  • Skin Irritation study
  • Genotoxicity
  • AMES test (BRMT)
  • Chromosomal aberration in CHO-K1 cells
  • Micronucleus assay
  • In vitro organ specific toxicity
  • Specific organ toxicity
  • Reproductive toxicity
  • Histopathology

Others

We offer other In vitro & in vivo services in

  • Hypercholesterolemia
  • Arthritis
  • Osteoporosis
  • Benign prostatic Hyperplasia
  • Spermatogenesis
  • Allergy
  • Antitussive
  • Psoriasis

PRODUCT DEVELOPMENT (F&D)

At Radiant Research, our goal is to support our clients business from the identification of lead molecule to formulation of product with an objective of development of bio-available, stable and optimal dosage form for specific a specific administration route.

At Radiant Research, our formulation and development services include,

  • Formulation development for NCE and polyherbal combinations
    Optimization of existing formulations
  • Novel formulations for improved delivery of existing dosage forms
    Controlled release and sustained release formulations
  • Evaluation of pre-formulation parameters
  • Evaluation of post-formulation parameters
  • Formulation stability analysis

SERVICES